Determination of “Static Dose” in Murine Thigh and Lung Infections
Determine cfu/thing in untreated controls and mice treated with 4-5 different total doses
Use nonlinear regression and modified Hill equation to estimate Emax (difference from untreated control), P50 (dose giving 50% of Emax) and slope (N) of dose-response relationship ?CFU = (Emax) DoseN/ DoseN + P50N
Calculate “static dose” Log “static dose” = [log (E/E-Emax)]f/N + log P50 , where E = control growth